This study aimed to assess vitamin D status and its association with iron status in young Iraqi children. A total of 95 infants and toddlers with iron deficiency (ages ranging from 6 to 24 months) and an equal number of 95 healthy subjects with normal hemoglobin (Hb) and sufficient ferritin level with matching age were included as a control group. A specially designed questionnaire was used to collect data. The cases were classified into iron deficiency (ID) and iron deficiency anemia (IDA) according to hemoglobin and ferritin levels. The cases and control groups were subdivided into vitamin insufficiency (VDI), vitamin D deficiency (VDD), and vitamin D sufficiency groups according to 25-hydroxyvitamin D [25(OH)D] levels. Young children with IDA have significantly lower serum levels of 25(OH) D compared with ID and control groups (p<0.05). According to iron status, VDI and VDD were present in 20% and 70% of IDA, 25.7% and 60%of ID, and 26.3% and 30.5% of control groups, respectively, with a significant difference in vitamin D level (p<0.05) among studied groups. A significant positive correlation (p=0.000) was found between serum ferritin level and 25(OH) D level in studied patients. Young children with severe iron deficiency have a higher prevalence of vitamin D deficiency, and there was a significant positive correlation between serum ferritin level and 25(OH) D levels among studied children.