A hospital information system is used to support a wide range of operations and activities in the hospital. This study was conducted to determine the factors affecting hospital information system acceptance by users.

A cross-sectional, descriptive, analytic study was performed in 2018. The study population included 550 users of the system. The data were collected using a questionnaire and analyzed using the SPSS software.

A significant moderate positive correlation was found between hospital information system acceptance and perceived usefulness (r = 0.54, P < 0.01), perceived ease of use (r = 0.41, P < 0.01), human factors (r = 0.46, P < 0.01) and technological factors (r = 0.54, P < 0.01). A significant weak positive correlation was detected between the acceptance of the hospital information system and organizational factors (r = 0.35, P < 0.01).

Perceived usefulness of the system, social influence, system quality, perceived ease of use of the system, and top managers’ supports had the most substantial influence on the users’ intention to accept a hospital information system. User education, preparation of guidelines suited to the user specialty or department, incorporating users’ work needs into the capabilities of the hospital information system, and improving the system to an ideal level are important considerations.