This study aimed to examine the effect of inter-dental abutment distance on the accuracy of digital and conventional impression methods. Five maxillary and mandibular models were prepared with different inter-dental abutment distances. Digital scans were obtained using an extraoral laboratory scanner as reference data. Each group was scanned 8 times using the intra-oral scanner for the digital method. For the conventional impression method, 8 additional silicone impression material was used to generate the stone casts from each group. Then casts were scanned. In the next step, stereolithography (STL) data was exported from the scans. The STL files were super-imposed on the reference scans using 3shape dental designer software to make the measurement. Kolmogorov-Smirnoff was used to determine if the data were normally distributed. In the digital impression method, as the abutment distance increased, the accuracy decreased. Various inter-dental abutment distances in digital groups showed significant differences (p=0.016) in impression accuracy, while the difference among conventional groups was not statistically significant (p=0.822). In the digital method, the mean inter-dental abutment between the 4-5 and 3-7 groups, 4-6 and 3-7 groups had a significant difference (p<0.05). However, the conventional method revealed no significant differences (p>0.05) between groups. In conclusion, when the inter-dental abutment distance exists and is surrounded by soft tissue, the possibility of error in the digital impression method is higher than in the conventional impression method.