2011, Volume 4, Issue 2, pp 172 – 177

Collagen–based scaffolds for skin tissue engineering


Special Issues

Authors and Affiliations

Correspondence to:Alexandra Gaspar, National Institute of Research and Development for Biological Sciences Bucharest, 296 Splaiul Independentei Street, P.O. Box 17–16, Romania, Phone/Mobile: 40 , e-mail alex.gaspar@yahoo.com


The aim of this study was to obtain four collagen based porous scaffolds and to assess their in vitro biocompatibility and biodegradability in order to use them for skin tissue engineering. We have prepared four variants of collagen–based biodegradable sponges by liophilization of type I collagen solution and three variants of collagen–agarose mixture in different ratios 2:1 (A), 1:1 (B) and 1:2 (C). These scaffolds had microporous structure with a higher than 98% porosity and a reduced biodegradation after their exposure to UV radiation. The incorporation of agarose into the collagen scaffolds has improved their structural stability. In vitro biocompatibility testing for the four types of sponges was performed on a stabilized fibroblast cell line and showed that both cell viability and morphology were not altered by collagen and collagen–agarose variants A and B sponges. These three porous sponges demonstrated potential for future application as cell scaffolds in skin tissue engineering.


About this article

PMC ID: 3124265
PubMed ID: 21776301

Article Publishing Date (print): 15-05-2011
Available Online: 25-05-2011

Journal information

ISSN Printing: 1844-122X
ISSN Online: 1844-3117
Journal Title: Journal of Medicine and Life

Copyright: © 2020 Carol Davila University Press. Romania

Copyright License: Open Access

This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use and redistribution provided that the original author and source are credited.